569 Hypoxic response of the expression of TGF-β2 and BMP4 in human dermal papilla cells

The structure of the lower part hair follicle undergoes cyclic formation and decay. During anagen, is surrounded by capillary blood vessels that supply oxygen nutrients. However, disappear during catagen, concentration in expected to decrease significantly. There little information on response human DPCs hypoxic condition follicle. In this research, we evaluated its influence expression TGF-β2 BMP4 dermal papilla cells (DPCs), which are command center cycle. study, immortalized were used. For creation hypoxia condition, cultured 10% FBS-DMEM with cobalt chloride. Because chloride inhibits prolyl hydroxylase, hypoxia-inducible factors (HIFs) not degraded even normoxia conditions. other words, addition into medium equivalent same as After cultivation, RNA isolation cDNA synthesis performed. Changes levels genes confirmed real-time PCR. When incubated DMEM chloride, expressions HIF-1α, HIF-2α, phosphoinositide-dependent protein kinase 1 (PDK1), L-type amino acid transporter (LAT1) increased. PDK1 one specific target gene for LAT1 HIF-2α. These results suggest can via HIF expression. And, work under DPCs. Moreover, when also It well known a catagen-inducing factor, telogen-maintaining factor. responsible system might it possible upregulated act maintain telogen state.